anti cd4 biotin  (Miltenyi Biotec)

Journal: Chinese Medical Journal

Article Title: FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway

doi: 10.1097/CM9.0000000000003493

Figure Lengend Snippet: MLR results of the four DC subsets isolated from GALTs of BALB/c mice. Among these four DC subsets, CD103 + CD11b + DCs and CD8α − CD11b + DCs presented the immune suppression ability compared with the control group (CD4 + T-cell cultured alone). * P <0.05, † P <0.01. DCs: Dendritic cells; GALTs: Gut-associated lymphoid tissues; MLR: Mixed lymphocyte reaction; ns: Not siginificant.

Article Snippet: CD4 + T-cells and CD4 + CD25 + T-cells were isolated using the CD4 + CD25 + Regulatory T Cell Biotin-Antibody Cocktail and the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol.

Techniques: Isolation, Control, Cell Culture

Journal: Chinese Medical Journal

Article Title: FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway

doi: 10.1097/CM9.0000000000003493

Figure Lengend Snippet: Percentage of CD25 + Foxp3 + T-cells analyzed by FC and results of cell migration. (A) Representative images of DCregs migration in the four groups (Crystal violet staining, Bars: 200 μm). (B) Comparison of the average migrated cell numbers of the four groups. Bars show the mean of migrated cell number selected randomly from five fields of view from each group, while the points show the migrated cell number of each field. Relative migration rate = Average number of migrated cells in experimental group/control group ×100%. * P <0.01, † P <0.001, by one-way ANOVA multiple comparisons. (C) The FACS plots showing the percentage of CD25 + Foxp3 + T-cells in each group. ANOVA: Analysis of variance; DCregs: Regulatory dendritic cell; FACS: Fluorescence-activated cell sorting; FC: Flow cytometry.

Article Snippet: CD4 + T-cells and CD4 + CD25 + T-cells were isolated using the CD4 + CD25 + Regulatory T Cell Biotin-Antibody Cocktail and the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol.

Techniques: Migration, Staining, Comparison, Control, Fluorescence, FACS, Flow Cytometry

Journal: Chinese Medical Journal

Article Title: FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway

doi: 10.1097/CM9.0000000000003493

Figure Lengend Snippet: Results of percentage of CD25 + Foxp3 + T-cells analyzed by FC. Data shown are representative of two independent experiments gated on CD4 + T-cells. (A, B) The FACS plots showing the percentage of CD25 + Foxp3 + T-cells in each group. FACS: Fluorescence-activated cell sorting; FC: Flow cytometry.

Article Snippet: CD4 + T-cells and CD4 + CD25 + T-cells were isolated using the CD4 + CD25 + Regulatory T Cell Biotin-Antibody Cocktail and the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol.

Techniques: Fluorescence, FACS, Flow Cytometry

Journal: Chinese Medical Journal

Article Title: FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway

doi: 10.1097/CM9.0000000000003493

Figure Lengend Snippet: Percentage of CD25 + Foxp3 + T-cells and expression level of Notch1 and JAG1 . (A) Representative FACS plots showing the percentage of CD25 + Foxp3 + T-cells in each group. (B) The Western blotting results of Notch1 and JAG1 in each group. (C) The RT-qPCR results of Notch1 and JAG1 in each group. DCreg: Regulatory dendritic cell; FACS: Fluorescence-activated cell sorting; FLT3L: Fms-like tyrosine kinase 3 ligand; RT-qPCR: Quantitative real-time polymerase chain reaction; Treg: Regulatory T-cells.

Article Snippet: CD4 + T-cells and CD4 + CD25 + T-cells were isolated using the CD4 + CD25 + Regulatory T Cell Biotin-Antibody Cocktail and the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Fluorescence, FACS, Real-time Polymerase Chain Reaction

Journal: Chinese Medical Journal

Article Title: FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway

doi: 10.1097/CM9.0000000000003493

Figure Lengend Snippet: Percentage of DCreg, CD4 + T cell and Foxp3 + Treg of colon LP and MLN. (A) Representative FACS plots showing the percentage of CD4 + T cell and Foxp3 + Treg of colon LP and MLN of each group. (B) Representative FACS plots showing the percentage of DCreg (CD103 + DC11b + DC) of colon LP and MLN of each group. (C) Comparison of the percentage of DCreg, CD4 + T cell and Foxp3 + Treg of colon LP and MLN. Data were represented as mean ± SEM ( n = 5). * P <0.05, † P <0.01 by one-way ANOVA multiple comparisons. ANOVA: Analysis of variance; DC: Dendritic cell; DCreg: Regulatory dendritic cell; DSS: Dextran sulfate sodium; FACS: Fluorescence-activated cell sorting; FLT3L: Fms-like tyrosine kinase 3 ligand; LP: Lamina propria; MLN: Mesenteric lymph node; ns: Not significant; PBS: Phosphate buffered saline.

Article Snippet: CD4 + T-cells and CD4 + CD25 + T-cells were isolated using the CD4 + CD25 + Regulatory T Cell Biotin-Antibody Cocktail and the autoMACS Pro Separator (Miltenyi Biotec), following the manufacturer’s protocol.

Techniques: Comparison, Fluorescence, FACS, Saline